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BenQ 8550T Driver

For western blot analysis, the animals were sacrificed with excessive chloral hydrate 0. The eyeballs were dissected out, and the cornea, lens and sclera BenQ 8550T removed on an ice-cold plate.

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The slides were thawed and washed three times for 10 minutes with 0. The sections were then incubated with mouse anti-rat glial fibrillary acidic protein monoclonal antibody 1: Finally, the sections were mounted in 1: BenQ 8550T controls performed with the secondary antibody alone showed no staining. All images were captured using a laser scanning fluorescence microscope Nikon 80I, Tokyo, Japan.

The retinal tissues were homogenized by an ultrasound homogenizer at a ratio of 1: Protein quantification was performed using bicinchoninic acid protein BenQ 8550T kit CWBIO Technology in duplicate and the results were averaged.

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The blots were subsequently incubated for 1 hour at room temperature with a BenQ 8550T peroxidase-conjugated AffiniPure goat anti-mouse IgG 1: Nonspecific staining was not detected when the primary antibodies were excluded. Two sections were selected from each retina.

Regulatory effects of inhibiting the activation of glial cells on retinal synaptic plasticity

The average gray value was negatively correlated with the fluorescent intensity of synaptophysin immunoreactivity. BenQ 8550T positive area of synaptophysin immunoreactivity in the outer nuclear layer and outer plexiform layer was measured blindly using ImageJ software[ 34 ].

The positive area percentage of synaptophysin immunoreactivity relative to the total area of the outer plexiform layer and outer nuclear layer was obtained. BenQ 8550T western blot analysis, the integrated density value for the intensity of the glial fibrillary acidic protein-immunoreactive bands was quantified by Fluor Chem software Alpha Innotech Corporation, San Leandro, CA, USA.

One-way analysis of BenQ 8550T and the independent sample t -test BenQ 8550T used to analyze the data using SPSS Conflicts of interest: None declared. Peer review: Glial cells play important roles in the formation and remolding of retinal synapses.

The authors BenQ 8550T confirmed that the expression of synaptophysin in retina was affected when glial activation was inhibited. Europe PMC requires Javascript to function effectively. Recent Activity.

BENQ 8550T DRIVER

The snippet could not be located in the article text. This may be because the snippet appears BenQ 8550T a figure legend, contains special characters or spans different sections of the article.

Neural Regen Res. Corresponding author: Accepted Jan 4. This is an open-access article BenQ 8550T under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3. This article has been cited by other articles in PMC.

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